About the extraction of nucleic acids

Basic knowledge of nucleic acid

Nucleic acid is divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), among which RNA can be divided into ribosomal RNA (rRNA), messenger RNA (mRNA) and transfer RNA (tRNA) according to different functions.

DNA is mainly concentrated in the nucleus, mitochondria and chloroplasts, while RNA is mainly distributed in the cytoplasm.

In nucleic acids, purine bases and pyrimidine bases have conjugated double bonds, so nucleic acids have ultraviolet absorption characteristics. The ultraviolet absorption of DNA sodium salt is around 260nm, and its absorbance is represented by A260. It is in the absorption trough at 230nm, so ultraviolet spectroscopy can be used. The photometer is used for quantitative and qualitative determination of nucleic acids.

Nucleic acid is an amphoteric electrolyte, which is equivalent to a polyacid. A neutral or alkaline buffer can be used to dissociate nucleic acid into anions, which are placed in an electric field and move towards the anode. This is the principle of electrophoresis.

Principles and requirements of nucleic acid extraction and purification

1. Ensure the integrity of the primary structure of nucleic acid

2. Eliminate pollution from other molecules (such as eliminating RNA interference when extracting DNA)

3. There should be no organic solvents and high concentrations of metal ions that can inhibit enzymes in nucleic acid samples

4. Minimize macromolecular substances such as protein, polysaccharides and lipids as much as possible

Nucleic acid extraction and purification method

1. Phenol/chloroform extraction method

Invented in 1956, after phenol/chloroform treatment of cell crushing liquid or tissue homogenate, nucleic acid components mainly composed of DNA are dissolved in the aqueous phase, and lipids in the organic phase, and proteins are located between the two phases.

2. Alcohol precipitation method

Ethanol can eliminate the hydration layer of nucleic acid and expose the negatively charged phosphate groups. Positively charged ions such as NA﹢ can combine with the phosphate groups to form a precipitate.

3. Chromatography column method

The special silicon matrix adsorption material can specifically adsorb DNA, and RNA and protein can pass through smoothly, and then use high salt and low pH to bind nucleic acid, and low salt and high pH to elute to separate and purify nucleic acid.

4. Thermal cracking alkali method

Alkaline extraction mainly uses the topological difference between covalently closed circular plasmids and linear chromatin to separate them. Under alkaline conditions, denatured proteins are soluble.

5. Boiling cracking method

The DNA solution is heated to use the characteristics of linear DNA molecules to separate the DNA fragments from the precipitate formed by denatured proteins and cell debris by centrifugation.

6. Nano magnetic bead method

After the surface of superparamagnetic nanoparticles is improved and surface modified by nanotechnology, superparamagnetic silicon oxide nanomagnetic beads are prepared. The magnetic beads can specifically recognize and efficiently bind with nucleic acid molecules on the microscopic interface. Using the superparamagnetism of silica nanospheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, DNA and RNA are separated from samples such as blood, animal tissues, food, pathogenic microorganisms, etc. .

7. Other methods

In addition to the above-mentioned commonly used methods, there are multiple methods such as ultrasound, repeated freezing and thawing, enzymatic hydrolysis, and hypotonic lysis.

8. Types of nucleic acid extraction

1. Total RNA extraction

Of the total RNA, 75-85% is rRNA (mainly 28S-26S/23S and 18S/16S rRNA), and the rest consists of mRNA and small RNA with different molecular weights and nucleotide sequences such as tRNA, 5S rRNA, 5.8S rRNA, miRNA, siRNA, small nuclear RNA (small nuclear RNA, snRNA) and nucleolar small molecule RNA (small nuceolar RNA, snoRNA) and other components.

2. miRNA extraction

MicroRNAs (miRNAs) are small, highly conserved RNA molecules, such as small interfering RNAs (siRNAs), which regulate the expression of their homologous mRNA molecules by base pairing with them to prevent expression through various mechanisms. They have become a key regulatory agency for development, cell proliferation, differentiation and cell cycle.

3. Genomic DNA extraction

For gene structure and function research and genetic diagnosis, it is usually required that the length of the obtained fragment is not less than 100-200 kb. In the DNA extraction process, various factors that cause DNA fragmentation and degradation should be avoided as much as possible to ensure the integrity of the DNA and lay the foundation for subsequent experiments.

4. Plasmid extraction

The plasmid extraction method is to remove RNA, separate the plasmid from the bacterial genomic DNA, and remove proteins and other impurities to obtain a relatively pure plasmid.

The magnetic bead nucleic acid extraction kit designed and produced by Luoyang Aisen Biotechnology Co., Ltd. is packaged in a carton, containing a deep well plate and a stirring sleeve. The deep well plate contains magnetic beads, lysate, washing solution, binding solution and eluent, in addition to individually packaged proteinase K.

/by

Introduction to the use of diamond polishing pads

Diamond polishing pad is a flexible processing tool made of diamond as abrasive and combined with composite materials. The back is glued with Velcro cloth, which is glued to the joint of the grinding machine. It is used for grinding, stone, ceramic, glass, and floor tiles. Special-shaped processing of other materials, suitable for stone polishing, line chamfering, curved board and special-shaped stone processing, and can also be used for marble, coagulation, cement floors, terrazzo, glass-ceramics, artificial stone, floor tiles, glazed tiles, vitrification The special-shaped processing, repair and renovation of bricks have strong grinding power, good durability, good softness, clarity, and good gloss. It is an ideal stone grinding tool. The whole process is divided into rough grinding, fine grinding and fine grinding. After polishing in four stages, the processed stone has a gloss of over 90°.

6-inch-diamond-polishing-pads

The scope of use of diamond polishing pads

1. It can be applied to different places such as new and old concrete floors, terrazzo floors, diamond floors, etc., and different polishing meshes can be selected according to different needs of customers. The brightness of polishing can reach more than 90 degrees.
2. It is used for the renovation of industrial floors, warehouses, parking lots and other concrete floors or various aggregate hardener floors, especially the hardened dust-proof and sealed hardener floors, which can be flexibly matched with various hands according to needs and habits. For grinding or refurbishing, use polishing pads of different sizes for rough grinding, fine grinding, fine grinding and polishing.
3. For the grinding and polishing of ceramic tiles, the ceramic tile manufacturer is equipped with manual, automatic full polishing machines and half polishing machines for the full polishing and half polishing of microcrystalline tiles, glazed tiles and antique tiles. Smooth and matt are optional. The brightness value of the glossy surface can reach more than 90; it is used for the ground treatment and renovation of microcrystalline tiles and various ceramic tiles. It can be flexibly used with various hand grinders or refurbishing machines according to needs and habits.
4. It is used for the treatment and renovation of various floors and steps laid by granite, marble, and artificial stone slabs. It can be used with various hand grinders or refurbishing machines according to needs and habits.

 

Diamond polishing pad specifications

Specifications: 3 inches, 4 inches, 5 inches, 6 inches, 7 inches, 8 inches
Thickness: 2.5mm, 3.0mm, 3.5mm;
Granularity: 30#, 50#, 80#, 100#, 120#, 150#, 200#, 300#, 400#, 500#, 600#, 800#, 1000#, 1500#, 2000#, 3000#

Characteristics of diamond polishing pad

1. Unique resin bond, strong grinding force;
2. Fast polishing speed and high finish;
3. High degree of wear resistance and long service life;
4. There is no scratch or color left on the stone after grinding and polishing (not easy to stain).

 

Diamond polishing pads are made of high-quality diamond micropowder and resin bond materials, with exquisite technology and advanced production technology. They are mainly used for the polishing and polishing of various shapes of natural stones or artificial stones. They can also be used for decorative stone cleaning, trimming and Update. It can be flexibly matched with various hand grinders, floor refurbishing machines, ceramic polishing machines, angle grinders, floor grinders, etc. according to customer needs and habits.

/by

What is the reason for the chromatic aberration of the stone of the floor renovation sheet?

Now everyone’s living conditions are getting better and better, and they are beginning to pay attention to the things around them slowly. Among the products we produce, some products need to be polished because of their rough appearance. At this time, our polishing pads are brought into play. , There are many polishing pads around us, such as diamond polishing pads, concrete polishing pads, resin polishing pads, etc. These polishing pads have a very large effect and have different applications. Dry polishing pads can be polished without water. , Water-based polishing pads need to be polished with water. In addition, we now have floor refurbishing films. Next, let’s talk to the polishing pad manufacturers to learn about floor refurbishing films.

Polishing-pad-size

The determination of the color of the stone in the floor renovation film should be a summary of multi-disciplinary such as architectural aesthetics, environmental science, humanistic psychology and economics, which is also the key for the designer to fully embody his design thinking and ideas. Of course, when using stone as decoration materials, color difference is a problem that cannot be ignored. Whether it is granite, marble or sandstone when selecting materials, there is a problem of color difference. Pay special attention when paving the same type on the same plane with a larger area.

Causes of color difference in stone

5-inch-diamond-polishing-pads

The stone blocks come from the initial mining layer of the mine, from different pit openings or different levels; after the stone processing, the row coding is not carried out according to the number and batch of the blocks; the stone paving does not follow the code list provided by the supplier And batch number. The introduction of floor refurbishment tablets Because of the reserves and ore bodies, the degree of color difference reflected in the detailed stone types is different. Some types of stone types close to 1,000 square meters or tens of thousands of square meters will not cause problems, while some types are several One hundred square meters cannot be guaranteed. Therefore, when choosing the type and color of stone, we must think about it. Large-scale plans can go to mines, factories, or existing cases to conduct on-site investigations.

6-inch-diamond-polishing-pads

From the above, we can know that the floor refurbishment sheet plays an important role in our floor refurbishment process. The price of the floor refurbishment sheet is relatively cheap, and it is very suitable for the use of the floor. When we refurbish the floor, we must pay attention to avoiding damage to the internal structure of the floor. We can only handle the surface of the floor. You need to be cautious when refurbishing. You can add some cleaners if necessary. I believe you will have a new style of floor after use, which will bring you a sense of comfort.

/by

Introduction of concrete polishing construction technology

With the continuous development of concrete materials and construction techniques, people’s performance requirements for concrete are not limited to compressive strength, but on the basis of strength, more attention is paid to the comprehensive indicators of concrete durability, deformation performance, aesthetics and decoration performance. Balance and coordination.

Polished concrete is a typical representative of concrete floors. It is a new type of high-strength, high-brightness inorganic floor, durable, super long life, and can be maintenance-free in the later stage. It is an all-round ground.

Concrete polishing features

In addition to the advantages of concrete polishing floor, it is easy to clean, low maintenance cost, and long service life. Its relatively high wear resistance coefficient makes it very suitable for public places with high traffic. Concrete polishing can also reduce allergens and inhibit the growth of mold. In addition, it has been proved through practice that high-reflectivity concrete polished floors can increase natural brightness and have a certain effect on reducing lighting energy consumption.

Concrete polishing construction technology

The early stage of the concrete polishing process includes four steps of grinding (rough grinding), repairing, curing, and polishing (fine grinding). Generally, the tools and steps used need to be further adjusted according to the actual situation to achieve the final design effect. The foreign leading ultra-fine polishing system also includes the “surface impermeability and anti-fouling protection process”. The effective protection can reach more than 15 years after the use of a special protective agent for concrete. During this period, only dry cloth or special cleaning pads are required to wipe. Floors such as ceramic tiles, etc. need to be maintained frequently with detergents, wax water, etc., and concrete polished floors are inexpensive and environmentally friendly.

(1) Grinding (rough grinding) refers to the use of metal diamond tools for grinding, which can remove fine pits, blemishes, colorants or other thin coatings on the ground (such as epoxy coating, self-leveling cement, PVC plastic ground, etc.) ) To prepare for the subsequent polishing process. According to different concrete floor conditions, the grinding process can usually be divided into three to four steps.

5 inch concrete polishing pads

(2) Repairing refers to the use of repairing agent to fill in the air bubbles, cracks, and fine cavities in the concrete.

(3) Curing refers to the process of applying curing agent on the surface of concrete to harden the concrete. The curing agent can not only penetrate into the small gaps of the concrete, tightly fill the pores, but also greatly increase the strength of the concrete and make it more durable, thereby greatly reducing the workload of later maintenance of the floor.

(4) Polishing (precision grinding) refers to the use of concrete polishing pads to polish the ground until the ground shows the expected gloss, which can be divided into three to five steps.

According to the ground conditions and gloss requirements, the maximum usable number of concrete polishing pads is 3,000. Concrete polishing pads of different meshes can make the surface appear elegant, pure matte, or high-reflective gloss.

13 inch concrete polishing pads

Although the concrete floor can be polished by wet grinding or dry grinding, because dry polishing is faster, easier to operate, and more environmentally friendly, dry polishing is currently one of the most commonly used polishing methods in the industrial field. Wet polishing requires water to cool the emery grinder and remove grinding debris from the machine. Since water can reduce friction like a lubricant, it can extend the life of the polisher. But the biggest disadvantage of wet polishing is that it produces a lot of mud. Dry polishing does not require the use of water, and the floor polishing machine used in dry polishing is equipped with a dust collector, which can effectively absorb dust particles.

Foreign advanced dust collection equipment usually has three-stage filtration equipment and pulse blowback device, which can filter out 99.9% of the dust above 1 micron (PM1). The collected dust can be used in brick making plants or mixing stations to replace fly ash Used in the production of concrete.

After the concrete polishing process is ground to remove the surface of the concrete, it can often express the natural color of the concrete better. The concrete mixed with premixed cement and specific or specialty stone aggregates (such as pebbles, granite, black basalt or ore, waste rock, etc.), after ultra-fine polishing, can show more color effects.

/by

How to maintain marble

Marble is hard material, which is one reason why people choose it as home decoration material. Although marble is strong, beautiful and elegant, it is a more sensitive material. You can usually use the term “externally soft and internally strong” to describe it.

Marble has a hard inner quality, but the polished appearance is extremely fragile and requires special maintenance. When you step on it, you should prevent marks from being left, and the objects placed on it should be handled gently. In response to the above problems, the best polishing pad manufacturers gave us a detailed introduction on the methods of marble maintenance, let’s take a look!

Marble

1. Water and wet substances

From the appearance, water does not pose a threat to the beautiful and elegant marble surface. In fact, it is deceived by the surface phenomenon. Water has enough power to destroy the beautiful marble sheet. The water or other damp substance mixed in the glass is enough to leave scale on the surface of the marble, and the scale will slowly penetrate the entire marble surface and leave stains that are difficult to wipe off.

A good way to deal with it is to prevent water droplets from dripping on the marble surface, but in reality it is often impossible to do so, so what you can do is to wipe off the water droplets in time, so as to prevent the progress of water from entering.

2. Acidic and alkaline substances

When cleaning the marble sheet, a neutral detergent should be used. Both acidic and alkaline detergents can easily corrode the surface of the marble.

Of course, it is not only detergent that will corrode the surface of the marble, like some acidic substances such as orange juice, lemon juice, soda, apple juice, tomato juice, wine, etc., will corrode the surface of the marble, and the PH Alkaline bleach will also corrode marble.

Granite

3. Scratches

There is no doubt that the internal quality of the refurbished marble is hard, but the smooth surface is very fragile. If the sole is stuck with sand, it is easy to leave scratches, and the bottom of the furniture will also give the marble surface if it is not placed protectively. Leave scratches, and drag objects on the marble floor.

The basic protection method is to shake off the gravel sticking to the sole of the shoe before stepping on the floor, put a protective layer on the furniture, try to avoid dragging objects on the marble floor, and don’t have things with sharp edges.

Of course, there are other substances that pose a threat to marble, but as long as you pay attention to the details mentioned above, it can bring many uses for your marble maintenance.

/by

Marble floor polishing pad polishing process

Because of the long-term use of marble, the brightness and gloss of the crystal surface of the marble surface will disappear, which will affect the overall decoration design of the home. When the brightness and gloss of the crystal surface of the marble disappear, what method can we use to make the marble crystal surface What about rejuvenating? Generally, in this case, we need to polish the marble to regain its brilliance.

Polishing process of marble floor polishing pad

First of all, the process of grinding the ground must be done. In the grinding process, 50#, 150#, 300# marble polishing pads need to be used in order for the grinding operation. After finishing the process of polishing the ground, the surface water is sucked dry by a water suction machine.

During construction, it is necessary to first polish the corners in place, and then do the grinding and corner processes at the same time, and make the ground absorb at the same time, so that the effect of the ground will show a better effect. When the floor is cured, the curing agent must be sprinkled after the floor is dried, and the floor must be kept moist for more than 4 hours. This part of the process is also the best way to prolong the life of the floor and improve the performance.

6-inch-diamond-polishing-pads

Ground fine grinding needs to be carried out again after the curing agent is dried, and use 500#, 1000#, 2000#, 3000# marble polishing pads in order to grind with water, and then use a water suction machine to suck out the surface water to keep it dry. Clean water should be used in the process, which is conducive to the next cleaning work.

The floor polished by the above process can restore the luster of the marble floor polishing pad, and the effect is the same as a new floor. The marble polishing pad also plays a very important role in the construction process, whether it is grinding or fine grinding. The construction personnel should choose the marble polishing pad for grinding according to different steps, so the quality of the polishing pad is very important.

In the processing of marble polishing pads, grinding is a method often used in the construction process. The use of grinding wheels of grinders to cut marble polishing pads has achieved the expected results.

5-inch-diamond-polishing-pads

(1) For materials with high hardness, it is generally used for processing. The hardness and heat resistance of the abrasive grains of the grinding wheel determine the effect of the marble polishing pad after processing.

(2) Stress and deformation of the workpiece, burns on the surface of the workpiece, etc. are also common problems in the processing process. The severe friction during the machining process causes the temperature of the grinding zone to gradually increase, thus causing the above-mentioned problems. Coolant plays a very important role here, adding a certain amount of coolant in the grinding, so that the grinding temperature can be reduced. In addition, the coolant also has critical functions for chip removal and lubrication.

(3) The elastic retreat of the “machine tool-grinding wheel-workpiece system” is also a problem that easily arises in the machining process. This is caused by the large radial force during grinding, making the actual depth of cut less than the nominal depth of cut. Therefore, before the end of the grinding operation, it is necessary to shut down the grinding without inserting the knife to increase the error rate of the operation.

(4) Some materials are determined by the characteristics of the grinding wheel and the grinder during the construction process. Uniform micro-cutting plays a vital role in the grinding process system, such as ap=0.001~0.005mm; grinding during the construction process Cutting can reach high speed operation, such as: v=30~50m/s; if you want to achieve higher machining accuracy, you can use the grinding method; sometimes it is necessary to use hydraulic transmission, such as; (IT6~IT5 ) And (Ra=0.8~0.2μm), grinding is also one of the most commonly used methods.

/by

Introduction of granite renovation technology

Granite is also called granite, and it is composed of quartz and mica. Its hardness value is about 6.5. If it is used for a long time, it will become weathered and wear out. Mica shedding is its most obvious feature.

At this time, there are a small amount of pits on the surface of the stone. If the pits increase, the brightness of the granite surface will be reduced and the original luster will be lost. When you touch it with your hand, you will feel bumpy again, and you need to restore the original stone with renovation. luster.

Due to the hard texture of granite, the use of chemical curing agents and abrasives to refurbish its surface is difficult to achieve the expected results. The use of stone refurbishing agents using physical grinding methods is better than using chemical products for refurbishing and grinding. Stone renovation agent, a full set of granite polishing pads, water suction machine, watering can, photometer, glass scraper, wiper and bucket are the equipment needed for granite renovation.

Spiral polishing pad

Granite working procedure

1. Set up a notice board at the work site. In order to prevent mud from splashing on the wall, use plastic or newspaper to paste the wall. After adding clean water to the stone refurbishing machine, after connecting the power supply, prepare to refurbish the discs and adjust the equipment to be at the same level as the ground. The next step is to prepare.

2. Installed on the refurbished disc needle plate, you should use a thick metal mouth disc. When grinding about 1 square meter, you must keep pushing and pulling to grind the stone cut part until it is smooth. When grinding, add moderate water. Don’t dry it, use a water suction machine to suck up and clean the ground at the same time.

3. Replace the thin metal mouth discs, grind them in order, and cut the genuine ones to eliminate rough marks. Always push and pull to grind, soak up the ground.

4. Press 50#, 200#, 400#, 800# granite polishing pads to refurbish and continue grinding.

5. Finally, use 1500#, 3000# granite polishing pads for polishing, and at the same time clean and dry the stone surface.

Granite stone renovation steps

Polishing-pad-size

1. Disease treatment

The surface of granite is eroded over time and is prone to disease. Such as: pigment infection, macula, etc., for this phenomenon, we must use professional agents to scrub.

2. Slit

Use a stone cutter to cut along the original seam, and then use a vacuum cleaner to clean up the stains in the gap.

3. Apply protective agent

The corrosion resistance of granite is not good, so it is best to brush several layers of protective agent on its surface. This protective agent can well prevent the granite from being damaged during use or renovation.

5 inch concrete polishing pads

4. Replenishing glue

Glue patching is the process of patching granite. You can also adjust the corresponding color of the marble glue according to your needs, and use a scraper to repair the glue; if the stone has cracks or potholes, you can also deal with it together.

5. Cut and polish

When we finish applying the marble glue, there will definitely be overflowing glue on the surface. At this time, we have to polish the surface with a grinder to remove the excess glue and polish until the seams can be clearly seen.

6. Coarse grinding

The previous step of polishing is for the joints, and this step of polishing is for the granite surface, polishing the dull surface to a new layer, and it must be smooth.

7. Fine grinding

Fine grinding is to carefully polish the granite stone in a targeted manner, especially the scratches left by the previous steps, will be polished off at this step.

8. Polishing

In order to restore the translucent granite to the past, polishing is a necessary step. When using a polishing machine, one should control the amount of water added, which is more conducive to the restoration of gloss.

9. Crystal hardening treatment

After polishing, the surface layer is very fragile, so we need to reinforce it for mirror finish. Crystal powder or crystallization agent can be used.

10. Clean the dust

After the previous procedures are completed, a large amount of dust will be left, and finally the construction site is cleaned.

/by

What are the epoxy floor repair methods?

Epoxy floor is a kind of floor product with very good decoration and function. Due to some reasons such as use, maintenance, construction, service life, etc., the epoxy floor will be damaged. Then when the epoxy floor is damaged , How do we repair it? First of all, follow the diamond polishing pad manufacturers to see what problems generally occur in epoxy floors. Then come to propose a specific repair plan.

1. Dimples, breakages, scratches, traces of roller rolling, etc. caused by customers’ lack of knowledge of floor use.

2. Peeling between layers caused by poor base foundation, hollowing, cracking caused by the stretching of expansion joints of the building structure, and hygroscopicity caused by improper waterproofing.

3. Damage caused by chemical corrosion, etc., mainly includes yellowing, blackening, and various stubborn stains.
4. Chalking and shedding caused by ultraviolet radiation.

5. Defects formed by the construction personnel or raw materials, such as dimples, pink particles, sweat stains, air dust pollution, flying insects, and reptiles.

In view of the above several epoxy floor problems, diamond polishing pad manufacturers have shared several most effective repair solutions.

Industrial-floor

1. Spray repair method

Advantages: Spraying method repair has the incomparable advantages of other methods, after repairing, the traces are small, the appearance is good, and the material is saved.

Disadvantages: The construction is difficult, and the requirements for the construction personnel are high, and professional spraying equipment is required.

2. Point complement method

Spot repair is the most commonly used method at this stage. Most construction workers use this method to repair damaged epoxy floors.

Advantages: The construction process is simple, easy to master, and the amount of raw materials is small.

Disadvantages: obvious repair marks, unsightly, and height difference.

Parking-lot-floor

3. Overall coverage

Advantages: beautiful and beautiful.

Disadvantages: high cost, only suitable for small spaces and areas with heavy chemical corrosion.
The construction process is similar to the new epoxy resin floor, so I won’t repeat it.

4. FRP construction method, etc.

Scope of application: long cracks such as expansion joints in building structures.

1. Basic treatment: Now cut the V-shaped opening at the crack, and use a vacuum cleaner to remove the floating dust.

2. Primer construction

3. Repair construction

4. Epoxy putty construction

The above are the common repairing solutions for epoxy floor shared by diamond polishing pad manufacturers. When there is a problem with the epoxy floor, you must first check, determine the problem, and then prescribe the right medicine to find a suitable repair method.

/by

6 steps of natural stone seamless treatment process

Natural stone is widely used as a paving material, and the noble, elegant, and luminous decorative fruits are deeply loved by people. In the stone maintenance system, there are continuously improving waxing, crystal surface treatment and other processes to ensure the brightness of the stone surface. The only difference is that the adjacent joints of the stone are not handled properly. The joints of the stone will be cut due to the lack of flatness of the stone or the stone itself. After the stone is used for a period of time, Irreversible pollution will occur in the joints, which will cause the joints to become black, and on the other hand, stains will penetrate into the pores of the stone to cause stone disease, which will seriously damage the overall beauty of the stone.

Stone seamless treatment The so-called seamless grinding treatment is to fill the gaps between the adjacent slabs that have been laid with a special caulking agent similar in color to the slabs, and then use industrial machinery and technology for grinding and polishing. . After being processed in this way, the stone slab will show the beauty of a large piece of stone that is not divided as a whole.

The construction process of stone seamless treatment generally has the following steps:

1. Clean the original filler between the stone gaps first, and then use the stone slitting machine to re-cut the center gap of the original stone installation neatly, so that the width difference of the gap between the stones is reduced to a low, and then use close to the stone. The colored marble glue fills it in. (Note: If the center seam has been done before, there is no need to re-sew).

2. Use marble glue close to the color of the stone and perfect the color of the marble glue to make it close to the color of the original stone to improve the overall feeling and achieve the ideal visual effect.

Spiral polishing pad

3. Fill the adjusted marble glue. Since the marble glue will shrink to a certain extent after solidification, the marble glue should be slightly higher than the stone level, so that it will not be filled multiple times.

4. Use polishing pads and polishing fillers to make it a horizontal surface with the stone, increase the overall feeling of the stone, and solve the problem of height between the gaps, and prevent the gaps from turning black again. Use a granite refurbishment machine in conjunction with a cutting edge grinding sheet to carefully polish the cutting edge to make it a flat integral with the stone.

5. Grinding and polishing: Use the used granite polishing machine to grind the stone from coarse to fine stone polishing pads of size 50-3000, so that the ground is bright and smooth as new.

5-inch-diamond-polishing-pads

6. Crystallization treatment: Use stone crystal powder and crystal agent to polish with crystal surface grinder. Under the weight of grinding, the stone and crystal powder will undergo a comprehensive physical and chemical reaction under the action of high temperature to form a dense and hard crystal protective layer. After crystallization, it can enhance the color and brightness, and can also achieve anti-skid, waterproof, oil-proof and other functions.

After the above process treatment, the gap will have good brightness, close to the color of the stone, and the overall feeling is strong. Moreover, because of the stain resistance, water resistance and oxidation resistance of the marble glue, there will be no black seams.

/by

What are the renovation and transformation processes of industrial floors?

The ground is frequently used in the building, so it is easy to be damaged. Due to the limitation of technology, many old grounds (such as epoxy, emery, terrazzo, floor tiles, cement floors, etc.) gradually exposed their defects and appeared different The problem of form and degree, so to a certain degree, needs to be refurbished. Diamond polishing pad manufacturers are coming to introduce you to several renovation construction techniques commonly used in industrial floor renovation.

The construction process of epoxy resin floor

6-inch-diamond-polishing-pads

1. Treatment of plain land: polishing, repairing, and dust removal according to the condition of the plain land

2. Epoxy primer: use epoxy primer roller coating with strong permeability and adhesion to enhance surface adhesion

3. Epoxy soil approved: According to actual needs, it shall be constructed several times, and the requirements shall be smooth and free of holes, and no batch of knife marks and sanding marks shall prevail

4. Epoxy topcoat: two coats of soluble epoxy topcoat or anti-slip topcoat

5. Construction is completed: You can only get on people after 24 hours, and you can press hard after 72 hours. (25℃ shall prevail, the low temperature opening time needs to be extended appropriately)

Construction technology of concrete sealing and curing agent floor (mirror type)

5-inch-diamond-polishing-pads

1. Grassroots treatment: before construction, clean up debris, oil stains, curing agents, etc. on the ground. Cement-based repair mortar was used to repair potholes and cracks on the ground and cured for 3 days.

2. Polishing the ground: the surface quality is poor and rough: install a rough diamond module on a terrazzo machine (7.5KW machine), add water to grind the ground, and then change the fine grinding head separately, and then grind the ground with the fine grinding head. The same process is water milling) until the ground is smooth. After grinding the ground, clean the ground with water and at the same time use a vacuum cleaner to suck up the sewage. For better, smooth new wear-resistant floors, you can directly apply concrete sealant curing agent.

3. Brush the concrete sealing curing agent: After the floor is cleaned, let the surface air dry, you can start to apply the concrete sealing curing agent. Use a roller or sprayer to evenly brush the curing agent on the ground. The average amount of coating is 10㎡/kg. If the base surface is poor and the permeability is strong, it must be painted twice after 1-2 hours; after about 1-2 hours (before the material is dry and shiny), spray some water on the ground to moisten the material, Use a broom or floor mop to scrub the surface to make the material penetrate into the ground, and repeat 2-3 times, then rinse the ground to remove the excess material on the surface.

4. Ultra-fine polishing (mirror fruit): In order to increase the gloss and brightness of the ground to achieve mirror fruit, 1.1KW high-gloss polishing machine can be used, and 500 mesh, 1000 mesh, 2000 mesh, and 3000 mesh diamond polishing pads can be attached to the disc. Water the super-fine polished floor.

Construction technology of wear-resistant floor

4-inch-diamond-polishing-pads

1. Removal of laitance: Use a machine equipped with a disc to slowly and evenly destroy the laitance on the surface of the single concrete.

2. Spreading material: Spread the specified amount of 2-3 wear-resistant material on the concrete surface in the initial setting stage. After the wear-resistant material absorbs water, the mechanical operation of installing the disc is carried out, and the wear-resistant material is hardened to a certain stage, and the wear-resistant material is spread twice.

3. Disc operation: After the wear-resistant material absorbs water, perform at least three mechanical operations to install discs. The speed of the machine depends on the hardening of the concrete. The mechanical trowel should be crisscrossed.

4. Surface finishing: The final finishing of the wear-resistant floor material is processed by mechanical trowel or manual trowel. When finishing, it should be crisscrossed. The angle of the iron plate and the speed of the machine should be adjusted according to the hardening of the ground surface. .

/by